NUCLEOFILL Strong 1x1,5ML

What is Nucleofill Strong?

Nucleofill Strong is a high-concentration tissue biostimulator from the Promoitalia Group SpA range (Milan, Italy) based on polymerized polynucleotides (PN) at a concentration of 25 mg/ml. This is more than three times the concentration of the sister product Nucleofill Soft Plus Eyes (7.5 mg/ml), which is designed exclusively for the periorbital area — Strong is formulated for intensive biostimulation of the face, neck, décolletage, hands and body, where the skin requires deeper regenerative intervention.

Nucleofill Strong addresses advanced signs of ageing: loss of volume and elasticity, deep wrinkles, skin laxity on the neck and décolletage — areas particularly susceptible to photoageing and collagen loss. Its mechanism of action is based on three parallel biological processes.

25 mg/ml PN – why does concentration matter clinically?

The concentration of the active substance in polynucleotide biostimulaors directly translates to the intensity and durability of biostimulation. Nucleofill Strong with 25 mg/ml PN is among the highest-concentration PN preparations available on the aesthetic medicine market:

• More PN molecules in the tissue = more binding points for fibroblast A2A receptors = more intensive collagen and elastin synthesis.
• Denser network of polymerized PN chains = more durable three-dimensional hydration scaffold in the extracellular matrix = longer-lasting hydration.
• Higher antioxidant potential = stronger free radical neutralisation = better protection against photoageing and oxidative degradation of collagen fibres.

This is why Nucleofill Strong is indicated where the skin requires intensive, not merely maintenance biostimulation — particularly in patients over 40 or with significant loss of skin density.

Triple-mechanism action of Nucleofill Strong

• Biostimulation — polymerized PN binds to adenosine A2A receptors on fibroblast membranes, triggering pathways responsible for synthesis of type I collagen and elastin. The higher 25 mg/ml concentration delivers more intensive receptor activation and faster dermal matrix densification.
• Antioxidation — PN is a potent free radical scavenger, neutralising reactive oxygen species (ROS) generated by UV radiation, environmental pollutants and metabolic processes. Antioxidant protection slows degradation of collagen and elastin fibres — a fundamental role in photoageing prevention.
• Iso-osmotic ECM hydration — the polymerized PN network forms a three-dimensional matrix retaining water in the extracellular matrix iso-osmotically — in harmony with tissue physiology, without the risk of excessive swelling associated with HA fillers.

Nucleofill Strong vs Nucleofill Soft Plus Eyes – which to choose?

• Nucleofill Soft Plus Eyes (7.5 mg/ml) — lower concentration, formulation dedicated exclusively to the periorbital area (thinnest skin, 30G needle). Gentle biostimulation, ideal for thin and sensitive skin around the eyes.
• Nucleofill Strong (25 mg/ml) — more than 3× higher concentration, for face, neck, décolletage, hands and body. Intensive biostimulation for skin requiring deeper intervention or with significant loss of elasticity.

Both products can be used in the same patient — Strong for the face and neck, Eyes for the periorbital area — as part of a comprehensive rejuvenation protocol.

Treatment results

• lifting and firming of facial, neck and décolletage skin,
• reduction of deep wrinkles and folds,
• improved skin elasticity and dermal density,
• stimulation of type I collagen and elastin synthesis,
• deep hydration of the extracellular matrix,
• improved radiance and skin tone,
• antioxidant protection — free radical neutralisation,
• improved skin quality of the neck and décolletage,
• rejuvenation of hand skin — improved hydration and density,
• reduction of chronological and photoageing signs.

Indications and treatment areas

• loss of skin elasticity and tone,
• deep wrinkles and nasolabial folds,
• skin laxity on neck and décolletage,
• ageing hand skin,
• advanced photoageing,
• skin requiring intensive biorevitalisation — patients over 40,
• complementing and maintaining results after lifting treatments,
• preventive and corrective skin rejuvenation.

Treatment areas: face, neck, décolletage, hands, body. Product is not intended for the periorbital area — use Nucleofill Soft Plus Eyes for that zone.

Treatment protocol

• Standard cycle: 2–4 sessions at 14–21 day intervals.
• Maintenance treatments: 1–2 times a year or as individually indicated.
• Injection techniques: nappage, serial micro-injections, linear technique — with the 30G 1/2" needle included in the pack.
• Volume per session: 1 pre-filled syringe of 1.5 ml.
• The detailed protocol should be determined by a qualified specialist, adapted to the treatment area and patient skin condition.

Composition and package contents

• Active substance: polymerized polynucleotides (PN) — 25 mg/ml
• Form: gel for intradermal injections
• Contents: 1 × sterile single-use pre-filled syringe 1.5 ml + 2 sterile single-use needles 30G 1/2"
• EAN: 8032880424760
• Manufacturer: Promoitalia Group SpA, Via San Gregorio 44, 20124 Milan, Italy

Contraindications

• pregnancy and breastfeeding,
• active infections or skin inflammation at the injection site,
• autoimmune diseases,
• hypersensitivity to product components (fish allergy is a relative contraindication — PN is derived from salmon/trout),
• blood clotting disorders or anticoagulant medication (medical consultation required).

FAQ – questions and answers

How does Nucleofill Strong differ from Nucleofill Soft Plus Eyes?

The key difference is concentration and area of use. Nucleofill Soft Plus Eyes has 7.5 mg/ml PN and is intended exclusively for the delicate periorbital skin. Nucleofill Strong has 25 mg/ml PN (more than 3× higher) and is used on the face, neck, décolletage, hands and body — where the skin is thicker and requires more intensive biostimulation. Both products can be used in the same patient.

Is Nucleofill Strong the same as PDRN?

Not exactly. PDRN consists of short DNA fragments (50–1500 kDa) acting primarily through A2A receptor activation. Nucleofill Strong contains polymerized polynucleotides of significantly higher molecular weight — forming a three-dimensional gel network in the tissue that provides both receptor biostimulation and durable, physical hydration of the extracellular matrix.

How does Nucleofill Strong differ from an HA skinbooster?

HA skinboosters hydrate and fill mechanically — they do not stimulate the production of the patient's own collagen. Nucleofill Strong biostimulates fibroblasts to synthesise collagen and elastin, provides antioxidant protection and hydrates iso-osmotically. The effect is biologically deeper and more durable — the skin actively rebuilds its own structure rather than simply being filled with an external substance.

When are the results visible?

Improved hydration and radiance is visible after 1–2 weeks from the first session. Lifting, improved elasticity and wrinkle reduction build gradually — the full collagen biostimulation effect develops over 4–8 weeks after completing the cycle.

Can Nucleofill Strong be used on the neck and décolletage?

Yes — neck and décolletage are among the primary indications for Nucleofill Strong. These areas are particularly susceptible to photoageing and collagen loss, and are often overlooked in rejuvenation protocols. The high 25 mg/ml concentration provides intensive biostimulation appropriate for the thickness and condition of skin in these areas.

How many sessions are needed?

Typically 2–4 sessions at 14–21 day intervals. For advanced changes or large treatment areas (e.g. neck + décolletage + face), the practitioner may recommend additional sessions. Maintenance treatments 1–2 times a year allow the effect to be sustained long-term.

This product may only be used by doctors and cosmetologists trained in aesthetic medicine and holding qualifications to perform injections. By purchasing this product you confirm that you hold the required professional qualifications.

• What is Nucleofill Strong?
• 25 mg/ml PN – why does concentration matter clinically?
• Triple-mechanism action – biostimulation, antioxidation, hydration
• Nucleofill Strong vs Nucleofill Soft Plus Eyes – which to choose?
• Treatment results
• Indications and treatment areas
• Treatment protocol
• Composition and package contents
• Contraindications
• FAQ – questions and answers